Corona

Alarming New Study Suggests Coronavirus Is Man Made Bio-Weapon [WATCH]

Uncanny similarity of unique inserts in the 2019-nCoV spike protein to HIV-1 gp120 and Gag

 

Prashant Pradhan , Ashutosh Kumar Pandey , Akhilesh Mishra , Parul Gupta , Praveen

1111 Kumar Tripathi , Manoj Balakrishnan Menon , James Gomes , Perumal Vivekanandan* and

1Kusuma School of biological sciences, Indian institute of technology, New Delhi-110016, India. 2Acharya Narendra Dev College, University of Delhi, New Delhi-110019, India

* Corresponding authors- email: [email protected] [email protected]

Abstract:

We are currently witnessing a major epidemic caused by the 2019 novel coronavirus (2019- nCoV). The evolution of 2019-nCoV remains elusive. We found 4 insertions in the spike glycoprotein (S) which are unique to the 2019-nCoV and are not present in other coronaviruses. Importantly, amino acid residues in all the 4 inserts have identity or similarity to those in the HIV- 1 gp120 or HIV-1 Gag. Interestingly, despite the inserts being discontinuous on the primary amino acid sequence, 3D-modelling of the 2019-nCoV suggests that they converge to constitute the receptor binding site. The finding of 4 unique inserts in the 2019-nCoV, all of which have identity /similarity to amino acid residues in key structural proteins of HIV-1 is unlikely to be fortuitous in nature. This work provides yet unknown insights on 2019-nCoV and sheds light on the evolution and pathogenicity of this virus with important implications for diagnosis of this virus.

Introduction:

Coronaviruses (CoV) are single-stranded positive-sense RNA viruses that infect animals and humans. These are classified into 4 genera based on their host specificity: Alphacoronavirus, Betacoronavirus, Deltacoronavirus and Gammacoronavirus (Snijder et al., 2006). There are seven known types of CoVs that includes 229E and NL63 (Genus Alphacoronavirus), OC43, HKU1, MERS and SARS (Genus Betacoronavirus). While 229E, NL63, OC43, and HKU1 commonly infect humans, the SARS and MERS outbreak in 2002 and 2012 respectively occurred when the virus crossed-over from animals to humans causing significant mortality (J. Chan et al., n.d.; J. F. W. Chan et al., 2015). In December 2019, another outbreak of coronavirus was reported from Wuhan, China that also transmitted from animals to humans. This new virus has been temporarily termed as 2019-novel Coronavirus (2019-nCoV) by the World Health Organization (WHO) (J. F.- W. Chan et al., 2020; Zhu et al., 2020). While there are several hypotheses about the origin of 2019-nCoV, the source of this ongoing outbreak remains elusive.

The transmission patterns of 2019-nCoV is similar to patterns of transmission documented in the previous outbreaks including by bodily or aerosol contact with persons infected with the virus.

bioRxiv preprint first posted online Jan. 31, 2020; doi: http://dx.doi.org/10.1101/2020.01.30.927871. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.

Casesofmildtosevereillness,anddeathfromtheinfectionhavebeenreportedfromWuhan. This outbreak has spread rapidly distant nations including France, Australia and USA among others. The number of cases within and outside China are increasing steeply. Our current understanding is limited to the virus genome sequences and modest epidemiological and clinical data. Comprehensive analysis of the available 2019- nCoV sequences may provide important clues that may help advance our current understanding to manage the ongoing outbreak.

The spike glycoprotein (S) of cornonavirus is cleaved into two subunits (S1 and S2). The S1 subunit helps in receptor binding and the S2 subunit facilitates membrane fusion (Bosch et al., 2003; Li, 2016). The spike glycoproteins of coronoviruses are important determinants of tissue tropism and host range. In addition the spike glycoproteins are critical targets for vaccine development (Du et al., 2013). For this reason, the spike proteins represent the most extensively studied among coronaviruses. We therefore sought to investigate the spike glycoprotein of the 2019-nCoV to understand its evolution, novel features sequence and structural features using computational tools.

Methodology:
Retrieval and alignment of nucleic acid and protein sequences

We retrieved all the available coronavirus sequences (n=55) from NCBI viral genome database (https://www.ncbi.nlm.nih.gov/) and we used the GISAID (Elbe & Buckland-Merrett, 2017)[https://www.gisaid.org/] to retrieve all available full-length sequences (n=28) of 2019- nCoV as on 27 Jan 2020. Multiple sequence alignment of all coronavirus genomes was performed by using MUSCLE software (Edgar, 2004) based on neighbour joining method. Out of 55 coronavirus genome 32 representative genomes of all category were used for phylogenetic tree development using MEGAX software (Kumar et al., 2018). The closest relative was found to be SARS CoV. The glycoprotein region of SARS CoV and 2019-nCoV were aligned and visualized using Multalin software (Corpet, 1988). The identified amino acid and nucleotide sequence were aligned with whole viral genome database using BLASTp and BLASTn. The conservation of the nucleotide and amino acid motifs in 28 clinical variants of 2019-nCoV genome were presented by performing multiple sequence alignment using MEGAX software. The three dimensional structure of 2019-nCoV glycoprotein was generated by using SWISS-MODEL online server (Biasini et al., 2014) and the structure was marked and visualized by using PyMol (DeLano, 2002).

 

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1 Comment

1 Comment

  1. Samuel Hay

    February 11, 2020 at 10:06 am

    True, it is man made in a lab and it even has a patent!

    January 22, 2020

    From: Samuel Hay
    PREVENTION AND CURE FOR CORONA VIRUS
    Re: I HAD SARS

    As an ardent researcher in many different fields some discoveries over the years have purposely been hidden away to protect Big Pharma’s massive profits. Some of these discoveries, it utilized could, in addition to healing and creating an atmosphere of less suffering for millions of people worldwide, save our own Medicare and Medicaid programs.
    Getting right to it, first of all there is a solution called ‘silver colloid’ which quite simply is microscopic silver particles in water. The brand I used to cure my killer staph infection which had me in bed for three months, dying, only took ten days to completely cure. Living is a wetland area I later cured myself of Lyme Disease in two weeks using the same silver colloid. I can only recommend Mesosilver as my personal experience was with this particular brand.
    About forty years ago I discovered the properties of ozone in relation to fighting communicable disease. I had to build my own ozone machine. It and its replacements have run 24/7 for all this time. Although Big Pharma has most likely been the culprit in having the FDA issue a warning, Im 77 years old and healthy as a mule. No communicable disease in this household. When they first came on the market they were priced around $650.00 but today with mass production the average cost is around $100.00 and even less for smaller models which could be used in public bathrooms to eliminate all, I said “All” disease germs. Instantly kills the AIDS virus on contact for example and it would take at least 20 minutes in pure Clorox! Ozone will permeate every inch of homes/office.
    The ozone produced in the machine is a very small amount especially compared with a lightning storm. That lightning is what cleanses the atmosphere. Have you ever seen a sign that said, “Do not breathe while near a thunderstorm?” I don’t think so. Without a positive mentality to cure not just treat contagious disease, no clinical tests will ever be performed. If you were dying from a contagious repertory disease, would you want to give it a go? I absolutely would!
    Germs cannot, as with the silver, ever become immune and the Chinese are currently fearing a mutation of the germs creating a public health emergency there.
    In layman’s terms, the microscopic silver particles in water do not work the same way as antibiotics. When the bacteria or virus uses it to hydrate, it cuts their guts apart.
    The ozone produced by the machines is O3 allowing a very unstable electron to be pulled into the outer ring but is very unstable. When it comes in contact with other atoms it can be pulled away. In the event such nearby atoms are bacteria or virus, the germs are electrocuted on a microscopic scale.
    The essence of this exercise is to make public the potential of these two discoveries as preventative and curative properties. When I go out in large crowds I spray my throat and nostrils with the silver colloids in water. Simple as that. You can take the silver internally and also apply it externally to wounds.

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